Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line (AGS)

Sat Dec 10 01:57:42 2022

(2008) Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line (AGS). Iranian biomedical journal. pp. 7-14. ISSN 1028852X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Calprotectin is cytotoxic agent that its anti-tumor effects are governed through suppression of topoisomerase II; a key enzyme in apoptosis. In previous studies, cytotoxicity and apoptotic effects of calprotectin are shown on different cancer cell lines, but not human gastric cancer cell lines. In the present study, cytotoxicity and apoptotic effects of calprotectin on human gastric adenocarcinoma cancer cell line (AGS) were evaluated. Methods: The AGS cells were exposed to the different concentrations of calprotectin for 24, 48 and 72 hours. Cell proliferation was assessed using dimethylthiazol diphenyl tetrazolium bromide assay and dye exclusion tests. For evaluation of cytotoxic mechanism in calprotectin on AGS cells, flow cytometric analysis was performed. Results: Our results revealed that calprotectin induces growth inhibition of AGS in a dose- and time-dependent manner. Results of this investigation showed that sensitivity of AGS cells to cytotoxic effect of human calprotectin was highly remarkable. In addition, growth inhibitory effect of this cytotoxic agent mostly was governed through induction of apoptosis in the AGS cells. Conclusion: These findings indicated that calprotectin induces growth inhibition and apoptosis in the AGS cells.

Item Type: Article
Creators:
CreatorsEmail
Zali, H.UNSPECIFIED
Rezaei-Tavirani, M.UNSPECIFIED
Kariminia, A.UNSPECIFIED
Yousefi, R.UNSPECIFIED
Shokrgozar, M. A.UNSPECIFIED
Keywords: Apoptosis Growth inhibition Human calprotectin Human gastric adenocarcinoma (AGS) 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide calgranulin article cancer inhibition cell proliferation controlled study cytotoxicity dose time effect relation drug effect drug sensitivity flow cytometry human human cell stomach adenocarcinoma Adenocarcinoma Annexin A5 Cell Line, Tumor Humans Leukocyte L1 Antigen Complex Stomach Neoplasms
Divisions:
Page Range: pp. 7-14
Journal or Publication Title: Iranian biomedical journal
Journal Index: Scopus
Volume: 12
Number: 1
ISSN: 1028852X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1667

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