Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluation of a PCR based approach to study the relatedness among Shigella sonnei strains

Tue Jun 25 04:35:41 2024

(2009) Evaluation of a PCR based approach to study the relatedness among Shigella sonnei strains. Iranian Journal of Clinical Infectious Diseases. pp. 163-166. ISSN 17355109 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Infections caused by Shigella are a major cause of diarrheal disease in the developing and developed countries. The present study was conducted to apply and evaluate arbitrarily primed PCR (AP-PCR) for investigation of genetic relatedness among the strains of Shigella sonnei isolated from cases of acute diarrhea in Tehran. Patients and methods: Totally, 60 S. sonnei strains isolated from children hospitalized due to enteritis at five hospitals in Tehran during 2003 and two sporadic isolates recovered in 1984 were investigated. Molecular typing was performed by AP-PCR. Depending on the number and size of amplified DNA bands, the strains were clustered into AP-PCR profiles. Results: All strains of S. sonnei were typeable with this approach. AP-PCR generated nine indistinguishable bands ranged from 0.35 to 2.5 kbp in all studied strains. Only a single AP-PCR pattern was observed among the S. sonnei strains recovered in 2003. Two sporadic isolates recovered in 1984 showed different AP-PCR patterns compared to recent clinical isolates. Conclusion: Results suggest that a very homogeneous AP-PCR cluster types might be responsible for shigellosis caused by S. sonnei in Tehran in 2003. Further molecular analysis conducted on a larger selection of isolates could confirm our findings. ©2009 IDTMRC, Infectious Diseases and Tropical Medicine Research Center.

Item Type: Article
Creators:
CreatorsEmail
Ranjbar, R.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Dallal, M. M. S.UNSPECIFIED
Farshad, S.UNSPECIFIED
Keywords: Acute diarrhea AP-PCR Molecular epidemiology Shigella sonnei article bacterial strain bacterium isolation child controlled study enteritis hospitalization human major clinical study polymerase chain reaction
Divisions:
Page Range: pp. 163-166
Journal or Publication Title: Iranian Journal of Clinical Infectious Diseases
Journal Index: Scopus
Volume: 4
Number: 3
ISSN: 17355109 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1655

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