Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The effects of PTP-1B knockdown on glucose uptake and triglyceride levels in C2C12 skeletal muscle cells

(2010) The effects of PTP-1B knockdown on glucose uptake and triglyceride levels in C2C12 skeletal muscle cells. Iranian Journal of Diabetes and Lipid Disorders. ISSN 17267544 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Insulin resistance is the central defect in obesity and type 2 diabetes. In Lipid accumulation and increased Protein Tyrosine Phosphatase-1B (PTP-1B) gene expression have been reported in muscle insulin resistance. The aim of this study was to investigate the effect of PTP-1B knockdown on glucose uptake and triglyceride levels in C2C12 cells. Methods: Reducing the expression of PTP-1B in C2C12 myoblasts was performed using the plasmids containing the shRNA against PTP-1B gene. PTP-1B protein level was assessed by western blot. The rate of glucose uptake and the intracellular triglyceride levels were evaluated in the PTP-1B knockdown and normal cells. Results: PTP-1B protein level in PTP-1B knockdown C2C12 cells decreased by 58 compared to the normal cells. Insulin-stimulated glucose uptake was decreased by palmitate in both the control and knockdown cells, whereas PTP1B knockdown cells treated with 0.5 and 0.75mM palmitate remained sensitive to the insulin with about 2.5 and 3-fold, respectively as well as increase in glucose uptake compared to the control cells. Treatment of the cells with 0.5 and 0.75mM palmitate resulted in 1.25 and 1.42 fold increase in triglyceride levels, respectively in the knockdown cells compared to the control cells. Conclusion: The results of this study suggest that increased insulin sensitivity in PTP-1B knockdown cells can be partly attributed to increased triglyceride levels within the muscle cells. Thus, reduced PTP-1B gene expression can be a potential therapeutic target for treating insulin resistance, type 2diabetes and metabolic syndrome.

Item Type: Article
Keywords: Glucose uptake Insulin resistance Palmitate Protein tyrosine phosphatase-1B Type 2 diabetes glucose palmitic acid protein tyrosine phosphatase short hairpin RNA triacylglycerol article cell line cell strain C2C12 gene expression glucose transport insulin sensitivity myoblast skeletal muscle triacylglycerol blood level
Divisions:
Journal or Publication Title: Iranian Journal of Diabetes and Lipid Disorders
Journal Index: Scopus
Volume: 9
ISSN: 17267544 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1640

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