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Ilam University of Medical Sciences

Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran

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(2011) Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran. Experimental Parasitology. pp. 375-380. ISSN 00144894 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

In Iran, three species of Leishmania have been incriminated as the causative agents of human leishmaniasis, Leishmania (L.) major, Leishmania tropica, and Leishmania infantum. Rhombomis opimus have been incriminated as a principal reservoirs of the parasitic protozoan Leishmania major, the causative agent of rural zoonotic cutaneous leishmaniasis (ZCL) in Iran. Rodents captured and examined to find Leishmania species using conventional methods including direct impression smear and microscopic observation inoculation samples to Balb/c and culture in NNN medium. Also molecular method was employed to detect Leishmania in rodents by amplifying a region of the ribosomal RNA amplicon of Leishmania (ITS1-5.8S rRNA-ITS2) using Nested PCR. Leshmania species were specified by DNA sequences. 36 (38.3) of R. opimus were Leishmania positive using at least one conventional methods. Many more ITS-rDNA fragments were amplified from R. opimus but only 65 out of 74 PCR products contained enough DNA for direct sequencing or readable sequences. The PCR assays detected in Iranian R. opimus not only Leishmania major in 59 (79.7) rodents but also Leishmania turanica in 6 (8.1) rodents, another parasite of the great gerbil. These parasites were found in Turkemen Sahara, North East of Iran, in a focus of rural (ZCL). L. major and L. turanica in R. opimus firmly identified from Turkemen Sahara. Nine rodents with Leishmania infections unidentified which some were unreadable sequences, these could be mixed infections of L. major, L. turanica, Leishmania gerbilli sensu lato and Leishmania close to L. gerbilli or a related species reported in sandflies previously from this location. The haplotypes of L. major and L. turanica were found to be identical to that of isolates of L. major and L. turanica from Iran and in GenBank elsewhere. R. opimus is probably the key reservoir in this ZCL focus because of its abundance and its infection rates with both L. major and L. turanica. © 2011 Elsevier Inc.

Item Type: Article
Creators:
CreatorsEmail
Mirzaei, A.UNSPECIFIED
Rouhani, S.UNSPECIFIED
Taherkhani, H.UNSPECIFIED
Farahmand, M.UNSPECIFIED
Kazemi, B.UNSPECIFIED
Hedayati, M.UNSPECIFIED
Baghaei, A.UNSPECIFIED
Davari, B.UNSPECIFIED
Parvizi, P.UNSPECIFIED
Keywords: Diagnostic nested PCRs Iran ITS-ribosomal DNA Leishmania major Leishmania turanica Rhombomis opimus Zoonotic cutaneous leishmaniasis DNA fragment ribosome RNA amplicon article controlled study culture medium DNA sequence geographic distribution gerbil host parasite interaction Leishmania Leishmania infantum Leishmania tropica microscopy nonhuman parasite isolation polymerase chain reaction priority journal sequence homology species identification Animals Disease Reservoirs DNA, Ribosomal Spacer Gerbillinae Humans Leishmaniasis, Cutaneous Mice Mice, Inbred BALB C RNA, Ribosomal, 5.8S Zoonoses Leishmania gerbilli Phlebotominae Protozoa Rhombomys opimus Rodentia
Divisions:
Page Range: pp. 375-380
Journal or Publication Title: Experimental Parasitology
Journal Index: Scopus
Volume: 129
Number: 4
Identification Number: https://doi.org/10.1016/j.exppara.2011.08.020
ISSN: 00144894 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1618

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