Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

mazE Antitoxin of Toxin Antitoxin System and fbpA as Reliable Targets to Eradication of Neisseria meningitidis

Wed Feb 28 13:37:59 2024

(2018) mazE Antitoxin of Toxin Antitoxin System and fbpA as Reliable Targets to Eradication of Neisseria meningitidis. Current Pharmaceutical Design. pp. 1204-1210. ISSN 1381-6128

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Abstract

Background: Neisseria meningitidis is considered as a dangerous pathogen threatening human health. Nowadays, the new drug target is focused. Toxin antitoxin (TA) system is recently identified as an antimicrobial drug target. Also, in N. meningitidis, iron-uptake system could be an interesting target for drug discovery. Methods: In this study, fbpA and mazE genes were chosen as new antimicrobial targets and treated with antisense peptide nucleic acid (PNA). Firstly, they were evaluated by bioinformatics and then analyzed by experimental procedures. Secondly, the functionality was evaluated by stress conditions. Results: Our results interestingly demonstrated that when fbpA and mazE loci of N. meningitidis were targeted by antisense PNA, 8 mu M concentration of fbpA-PNA as well as 30 mu M concentration of mazE-PNA inhibited the growth of N. meningitides and were found to be bacteriostatic, whereas 10 mu M concentration of fbpA-PNA showed bacteriocidal activity. Conclusion: Our findings demonstrated the bactriocidal activity of fbpA-PNA and bacteriostatic activity of mazE-PNA. Therefore, mazE and fbpA genes should be potent antimicrobial targets but further analysis including in vivo analysis should be performed.

Item Type: Article
Creators:
CreatorsEmail
Maleki, A.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Badakhsh, B.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Keywords: Neisseria meningitidis fbpA mazE mazF drug discovery iron-uptake system peptide nucleic-acids gene-expression antisense inhibition pathogenic neisseria messenger-rna bacteria india resistance emergence Pharmacology & Pharmacy
Divisions:
Page Range: pp. 1204-1210
Journal or Publication Title: Current Pharmaceutical Design
Journal Index: ISI
Volume: 24
Number: 11
Identification Number: https://doi.org/10.2174/1381612824666171213094730
ISSN: 1381-6128
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/157

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