Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran

Sun Aug 14 03:05:56 2022

(2013) The occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran. Iranian journal of microbiology. pp. 108-112. ISSN 20083289 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is of increasing clinical concern specially in children worldwide. The aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing Shigella spp. in Tehran, Iran. Materials and Methods: The study included all Shigella isolates recovered from pediatric patients aged less than 12 years admitted to a major pediatric hospital in Tehran, Iran, from 2008 to 2010. Bacterial identification, antimicrobial susceptibility testing, extended spectrum β-lactamases (ESBLs) screening and confirmatory tests were performed according to the standard guidelines. Conjugal transfer experiments and plasmid analysis were also carried out. Polymerase chain reaction and sequencing were used to identify the genetic determinants responsible for ESBL production. Results: Four out of 55 Shigella isolates, including three S. sonnei and one S. flexneri, showed an ESBL-positive phenotype. Plasmid transfer of the ESBL phenotype was successful for the S. flexneri isolate only. By PCR and sequencing, one S. sonnei isolate tested positive for the CMY-59 gene, while the other two S. sonnei and the S. flexneri isolates tested positive for the blaTEM-1 and blaCTX-M-15 genes. Conclusion: We found the prevalence of ESBL producing Shigella isolates was higher than detection rates observed in many other countries. Our finding raise concerns about the dissemination of ESBL among the strains of endemic S. sonnei throughout the country, because this species is now the most frequently isolated Shigella species in Iran and shigellosis by such strains in the community can pose a significant threat to patients and presents a challenge for disease management.

Item Type: Article
Creators:
CreatorsEmail
Ranjbar, R.UNSPECIFIED
Ghazi, F. M.UNSPECIFIED
Farshad, S.UNSPECIFIED
Giammanco, G. M.UNSPECIFIED
Aleo, A.UNSPECIFIED
Owlia, P.UNSPECIFIED
Jonaidi, N.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Mammina, C.UNSPECIFIED
Keywords: Antibiotic resistance Esbls Shigella spp amoxicillin plus clavulanic acid ampicillin cefalotin cefixime cefotaxime ceftazidime ceftizoxime ceftriaxone chloramphenicol ciprofloxacin cotrimoxazole doxycycline extended spectrum beta lactamase gentamicin kanamycin nalidixic acid neomycin nitrofurantoin piperacillin streptomycin tetracycline ticarcillin tobramycin antibiotic sensitivity article bacterium identification bacterium isolation child gene sequence human major clinical study nonhuman nucleotide sequence plasmid polymerase chain reaction preschool child prevalence school child Shigella shigellosis
Divisions:
Page Range: pp. 108-112
Journal or Publication Title: Iranian journal of microbiology
Journal Index: Scopus
Volume: 5
Number: 2
ISSN: 20083289 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1566

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