Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel anticancer activity and anticancer mechanisms of Brassica oleracea L. var. capitata f. rubra

Tue Jun 25 12:07:04 2024

(2013) Novel anticancer activity and anticancer mechanisms of Brassica oleracea L. var. capitata f. rubra. European Journal of Integrative Medicine. pp. 450-464. ISSN 18763820 (ISSN)

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Aim of the study: The anticancer activity of red cabbage (RC) and the underlying mechanisms against human cervical and hepatocarcinoma cancer cells were explored in this study. Materials and methods: The cytotoxic activity of RC extract was assessed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assay. ELISA was used to detect the level of anticancer cytokines, TNFα and IFNβ, in the culture supernatants of cancer cells. Apoptotic cells were investigated by flow cytometry. The levels of apoptotic genes (Bax, BCL-2, Capsases 7-9), cell cycle regulatory genes (cyclin D, E, and A) and tumor suppressor proteins (p27, p21, and p53) were assessed by real time RT-PCR. Results: The cytotoxic effect of the extract on normal human cells was significantly different from its effects on HeLa and HepG2 cells, 251.28. ±. 4.3, 23.38. ±. 1.87, and 28.66. ±. 2.85. mg/ml, respectively. The selectivity index (SI) was 10.88. ±. 0.82 for HeLa and 8.93. ±. 0.81 for HepG2 cells. Increased levels of TNFα, but not IFNβ were observed in the treated HeLa and HepG2 culture supernatants when compared with untreated cells. RC extract induced G0/G1 phase arrest in the cancer cells. The extract induced apoptosis, in a dose and time dependent manner, in treated HeLa and HepG2 cells while no observed apoptosis was found in untreated cells. Moreover, RC IC50 showed remarkable influence on the expression of the apoptosis-related genes in a positive and negative manner on both HeLa and HepG2 cells. Discussion/conclusion: RC extract could be considered as a novel anticancer agent providing new prospects for anticancer therapy using a natural product. © 2013.

Item Type: Article
Keywords: Anticancer Anticancer cytokines Apoptosis Brassica oleracea Cell cycle arrest 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide beta interferon caspase 7 caspase 8 caspase 9 cyclin A cyclin D cyclin E cytokine plant extract protein Bax protein bcl 2 protein p21 protein p27 protein p53 red cabbage extract tumor necrosis factor alpha tumor suppressor protein unclassified drug antineoplastic activity article cancer cell cancer cell culture cell cycle G0 phase cell cycle G1 phase cell cycle regulation cell function cell strain HepG2 controlled study cytokine production cytotoxicity drug selectivity enzyme linked immunosorbent assay female flow cytometry gene expression HeLa cell human human cell IC 50 liver cell carcinoma peripheral blood mononuclear cell priority journal protein function real time polymerase chain reaction red cabbage reverse transcription polymerase chain reaction supernatant uterine cervix cancer
Page Range: pp. 450-464
Journal or Publication Title: European Journal of Integrative Medicine
Journal Index: Scopus
Volume: 5
Number: 5
Identification Number:
ISSN: 18763820 (ISSN)
Depositing User: مهندس مهدی شریفی

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