Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Dynamics of bacteriophages as a promising antibiofilm agents

Mon Aug 8 17:56:51 2022

(2014) Dynamics of bacteriophages as a promising antibiofilm agents. Journal of Pure and Applied Microbiology. pp. 1015-1019. ISSN 09737510 (ISSN)

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Pseudomonas aeruginosa is an ubiquitous organism which has emerged as a major threat in the hospital environment. Overuse of antibiotics has also significantly increased the emergence of antimicrobial multiresistant bacteria. P. aeruginosa has an innate ability to adhere to surfaces and form virulent biofilms. Bacteriophage might represent one attractive solution to this problem. In this study, P.aeruginosa phage were utilized to Biofilm inhibition and remove.Sample collected from University sewage. Isolation was done according to Martha.R.J.Clokie protocol. Serial dilution prepared, then equally incubated with bacteria to investigate Biofilm inhibition potential. Biofilm formed base on Microplate Biofilm Assay. The effect of isolated phage investigated on biofilm remove of Pseudomonas putida, E.coli and Acinetobacter baumanii. P.aeruginosa biofilm had OD: 1.688 in 492n.m. Pure phage, 10-2 and 10-3 diluted phage decreased OD to 1.587, 1.341 and 1.461, respectively. Isolated phage dramatically decline OD of Biofilm of all strains.Phages have various affinity to attach to hosts, thereby it is supposed to phages compete for their receptors. Therefore it is supposed phages have most efficiency in optimum concentration to remove biofilm or growth inhibition.

Item Type: Article
Creators:
CreatorsEmail
Reza, A.UNSPECIFIED
Farid, A. J.UNSPECIFIED
Zamberi, S.UNSPECIFIED
Amini, R.UNSPECIFIED
Sajedeh, K.UNSPECIFIED
Ahmad, N.UNSPECIFIED
Hassan, A.UNSPECIFIED
Norkhoda, S.UNSPECIFIED
Morovat, T.UNSPECIFIED
Iraj, P.UNSPECIFIED
Keywords: Bacteriophage Biofilm Biofilm remove and biofilm inhibition Planktonic bacteriophage receptor Acinetobacter baumannii Article bacterial clearance bacterial growth bacterial strain bacterium isolation biofilm inhibition biofilm removal controlled study dilution Escherichia coli growth inhibition microbial population dynamics nonhuman Pseudomonas aeruginosa Pseudomonas putida sewage specific combining ability virus isolation water sampling
Divisions:
Page Range: pp. 1015-1019
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: Scopus
Volume: 8
Number: 2
ISSN: 09737510 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1545

Actions (login required)

View Item View Item