Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel identification of Leishmania major in Hemiechinus auritus and molecular detection of this parasite in Meriones libycus from an important foci of zoonotic cutaneous leishmaniasis in Iran

Sun Aug 14 02:59:39 2022

(2014) Novel identification of Leishmania major in Hemiechinus auritus and molecular detection of this parasite in Meriones libycus from an important foci of zoonotic cutaneous leishmaniasis in Iran. Journal of Infection and Public Health. pp. 210-217. ISSN 18760341 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: One of the well-known foci of zoonotic cutaneous leishmaniasis (ZCL) in Iran is Turkemen Sahara, which is located in north eastern Iran. ZCL is a disease of mammals, and humans can become infected as accidental hosts. Many researchers have argued that Rhombomys opimus is the only main reservoir host of ZCL in this region of the Golestan province. No other rodents or mammals are thought to host or have been reported to host Leishmania parasites in this region. This research was designed and developed to isolate, detect and firmly identify Leishmania parasites in mammals and rodents other than R. opimus. Methods: Wild mammals were caught from gerbil burrows. Leishmania parasites were detected to assess the infection of reservoir hosts in 2010. Each genomic DNA sample was screened for Leishmania infection via nested PCR and sequencing using the internal transcribed spacer ribosomal DNA (ITS-rDNA) identification protocol for parasites. Results: The greatest number of infections (8/19) were found in Meriones libycus. One in three infections was found in Hemiechinus auritus, and this is the first report of infection in this species. Only Leishmania major was definitively identified and unambiguously typed in M. libycus and H. auritus. The infection rates in these two wild mammals were not significantly different, and no other gerbil parasites were detected in M. libycus or H. auritus at our study site. Conclusions: Recent findings of Leishmania turanica in R. opimus and failures to detect L. turanica in M. libycus may be attributable to unidentified Leishmania infections in two M. libycus due to unreadable sequences. These cases may represent mixed infections by L. major and L. turanica. The assumptions that gerbil parasites can be co-infectors provide a starting point for the identification of the causative and potential parasites responsible for the frequent infections that are mainly mediated via sandfly vectors. © 2014 King Saud Bin Abdulaziz University for Health Sciences.

Item Type: Article
Creators:
CreatorsEmail
Rouhani, S.UNSPECIFIED
Mirzaei, A.UNSPECIFIED
Spotin, A.UNSPECIFIED
Parvizi, P.UNSPECIFIED
Keywords: Hemiechinus auritus Iran ITS-rDNA Leishmania major Meriones libycus Nested PCR genomic DNA internal transcribed spacer ribosome DNA small subunit ribosomal RNA protozoal DNA ribosomal spacer DNA animal experiment animal model anthropometry article controlled study DNA extraction genetic screening gerbil haplotype host infection rate mammal molecular biology nested polymerase chain reaction nonhuman nucleotide sequence parasite identification phylogeny polymerase chain reaction priority journal skin leishmaniasis zoonotic cutaneous leishmaniasis animal chemistry disease carrier DNA sequence epidemiology genetics hedgehog isolation and purification Leishmaniasis, Cutaneous parasitology veterinary Zoonoses Animals Disease Reservoirs DNA, Protozoan DNA, Ribosomal Spacer Gerbillinae Hedgehogs Sequence Analysis, DNA
Divisions:
Page Range: pp. 210-217
Journal or Publication Title: Journal of Infection and Public Health
Journal Index: Scopus
Volume: 7
Number: 3
Identification Number: https://doi.org/10.1016/j.jiph.2013.12.002
ISSN: 18760341 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1544

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