Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Molecular typing of salmonella Infantis clinical strains isolated in Tehran

Mon Aug 8 17:43:10 2022

(2014) Molecular typing of salmonella Infantis clinical strains isolated in Tehran. Journal of Military Medicine. pp. 17-22. ISSN 17351537 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Aims: Salmonella spp are among the most important food-borne pathogens both in humans and animals. The purpose of this study was to investigate the molecular types of S. infantis strains isolated in Tehran, Iran. Methods: Over a two year study, S. infantis strains isolated from various clinical samples in several hospitals of Tehran, were included in the study. Then, standard microbiological and serological methods were applied in order to identify isolated strains. Phenol-chlorophorm tech-nique also was used to extract bacterial DNA. The clonal relatedness between the isolated strains was studied by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Re-action (ERIC- PCR). Results: 40 isolated strains were identified as S. infantis which were further studied by ERIC-PCR. This technique produced 4 to 11 DNA bands. Besides, it divided all S. infantis strains into 8 genotype groups (I1-I8). I1 genotype group was the most common cluster (47.5). Conclusion: The results of study suggest that S. infantis strains recovered from clinical cases in Tehran, had a large genetic diversity. We also found the ERIC-PCR as a useful method for molecular typing of S. infantis isolated strains.

Item Type: Article
Creators:
CreatorsEmail
Ranjbar, R.UNSPECIFIED
Mirzaie, A.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Jonaidi, N.UNSPECIFIED
Keywords: Genetic Diversity Molecular Typing Salmonella Infantis
Divisions:
Page Range: pp. 17-22
Journal or Publication Title: Journal of Military Medicine
Journal Index: Scopus
Volume: 16
Number: 1
ISSN: 17351537 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1529

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