Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Use of TaqMan® real-time PCR for rapid detection of Salmonella enterica serovar Typhi

Sun Aug 14 02:43:55 2022

(2014) Use of TaqMan® real-time PCR for rapid detection of Salmonella enterica serovar Typhi. Acta Microbiologica Et Immunologica Hungarica. pp. 121-130. ISSN 12178950 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan® probes to detect Salmonella Typhi. TaqMan® real-time PCR assays were performed by designed primers and probe based on the staG gene for detecting S. Typhi. The specificity of the assay was evaluated on 15 Salmonella serovars. The analytical specificity was evaluated on 20 non-Salmonella microorganisms. The analytical sensitivity was assessed using decreasing DNA quantities of S. Typhi ATCC 19430. Finally the detection capability of the TaqMan® real-time PCR assay on isolates recovered from patients with Salmonella infections was compared to the conventional PCR assay. Only S. Typhi strain had positive results when subjected to the assay using Typhi-specific real-time PCR. No amplification products were observed in real-time PCR with any of the non-Salmonella microorganisms tested. The TaqMan® real-time PCR was more sensitive than the conventional PCR. In conclusion, we found that the easy-to-use real-time PCR assays were faster than conventional PCR systems. The staG-based TaqMan® real-time PCR assay showed to be specific and sensitive method for the safe and rapid detection of the S. Typhi.

Item Type: Article
Creators:
CreatorsEmail
Ranjbar, R.UNSPECIFIED
Naghoni, A.UNSPECIFIED
Farshad, S.UNSPECIFIED
Lashini, H.UNSPECIFIED
Najafi, A.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Mammina, C.UNSPECIFIED
Keywords: polymerase chain reaction Salmonella Typhi TaqMan® real-time PCR assay bacterial DNA genomic DNA article bacterium detection controlled study diagnostic test accuracy study DNA template intermethod comparison nonhuman polymerase chain reaction system real time polymerase chain reaction real time polymerase chain reaction system Salmonella enterica sensitivity and specificity typhoid fever Bacterial Typing Techniques DNA Primers Genes, Bacterial Humans Real-Time Polymerase Chain Reaction
Divisions:
Page Range: pp. 121-130
Journal or Publication Title: Acta Microbiologica Et Immunologica Hungarica
Journal Index: Scopus
Volume: 61
Number: 2
Identification Number: https://doi.org/10.1556/AMicr.61.2014.2.3
ISSN: 12178950 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1506

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