Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The mazEF toxin–antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis

Thu Dec 1 16:54:10 2022

(2015) The mazEF toxin–antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis. Drug design, development and therapy. pp. 2553-2561. ISSN 11778881 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The toxin–antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems are potent and sensitive targets in all E. faecium and E. faecalis strains. © 2015 Soheili et al.

Item Type: Article
Creators:
CreatorsEmail
Soheili, S.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Sekawi, Z.UNSPECIFIED
Neela, V. K.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Khosravi, A.UNSPECIFIED
Ramli, R.UNSPECIFIED
Hamat, R. A.UNSPECIFIED
Keywords: Enterococcus faecalis Enterococcus faecium Peptide nucleic acids Toxin–antitoxin system Virulence genes peptide nucleic acid antitoxin bacterial toxin virulence factor Article bacterial chromosome bacterial gene bacterial phenomena and functions bacterial strain bacterial virulence bacterium isolate controlled study efaAfm gene fms8 gene gene control gene expression gene location gene locus higBA gene mazEF gene nonhuman pilB gene plasmid sgrA gene stress toxin antitoxin system gene expression regulation genetics human isolation and purification pathogenicity Antitoxins Bacterial Toxins Gene Expression Regulation, Bacterial Humans Plasmids Virulence Factors
Divisions:
Page Range: pp. 2553-2561
Journal or Publication Title: Drug design, development and therapy
Journal Index: Scopus
Volume: 9
Identification Number: https://doi.org/10.2147/DDDT.S77263
ISSN: 11778881 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1452

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