Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Rosiglitazone, but Not Epigallocatechin-3-Gallate, Attenuates the Decrease in PGC-1α Protein Levels in Palmitate-Induced Insulin-Resistant C2C12 Cells

Fri Jun 21 14:02:52 2024

(2015) Rosiglitazone, but Not Epigallocatechin-3-Gallate, Attenuates the Decrease in PGC-1α Protein Levels in Palmitate-Induced Insulin-Resistant C2C12 Cells. Lipids. pp. 521-528. ISSN 00244201 (ISSN)

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Alteration of lipid metabolism is an important mechanism for the treatment of insulin resistance. PGC-1α, a key regulator of mitochondrial biogenesis and function, plays an important role in the improvement of insulin sensitivity by increasing fatty acids β-oxidation. In the present study, the effects of epigallocatechin-3-gallate (EGCG), an anti-obesity agent and enhancer of lipid catabolism, on PGC-1α protein expression was examined and compared with anti-diabetic drug rosiglitazone (RGZ). After differentiation of C2C12 myoblasts to myotubes, insulin resistance was induced by palmitate treatment. Then the expression of the PGC-1a gene and glucose uptake were evaluated before and after treatment with RGZ and EGCG. Palmitate treatment significantly decreased PGC-1α protein expression in C2C12 cells (P < 0.05). RGZ could restore the expression of PGC-1α in palmitate treated cells (P > 0.05), while EGCG had no significant effect on the expression of this gene (P < 0.05). RGZ and EGCG significantly improved glucose uptake (by 2- and 1.54-fold, respectively) in myotubes treated with palmitate. These data suggest that RGZ and EGCG both exert their anti-diabetic activity by increasing insulin sensitivity, but with different molecular mechanisms. This effect of RGZ, unlike EGCG, is mediated, at least partly, by increasing PGC-1α protein expression. © 2015 AOCS.

Item Type: Article
Keywords: C2C12 muscle cells Epigallocatechin-3-Gallate Insulin resistance PGC-1α gene Rosiglitazone epigallocatechin gallate palmitic acid peroxisome proliferator activated receptor gamma coactivator 1alpha 2,4 thiazolidinedione derivative antidiabetic agent antioxidant catechin creatine kinase glucose palmitic acid derivative Ppargc1a protein, mouse transcription factor animal cell antidiabetic activity Article gene expression glucose transport insulin sensitivity lipolysis mouse myoblast myotube nonhuman protein expression analogs and derivatives animal cell line cytology drug effects metabolism skeletal muscle cell Animals Antioxidants Hypoglycemic Agents Mice Muscle Fibers, Skeletal Myoblasts Palmitates Thiazolidinediones Transcription Factors
Page Range: pp. 521-528
Journal or Publication Title: Lipids
Journal Index: Scopus
Volume: 50
Number: 6
Identification Number:
ISSN: 00244201 (ISSN)
Depositing User: مهندس مهدی شریفی

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