Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In Vitro Evaluation of Achillea Millefolium on the Production and Stimulation of Human Skin Fibroblast Cells (HFS-PI-16)

Sat Jun 25 08:51:30 2022

(2015) In Vitro Evaluation of Achillea Millefolium on the Production and Stimulation of Human Skin Fibroblast Cells (HFS-PI-16). Medical archives (Sarajevo, Bosnia and Herzegovina). pp. 212-217. ISSN 0350199X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

AIM: In the present study, we aimed the effects of the hydroalcoholic extract of Achillea millefolium (HEAML) on human skin fibroblast cells (HSF-PI-16) proliferation, stimulation and growth properties. METHODS: Initially, using HSF-PI-16 monolayer culture, we created one line scratch method as an in vitro wound closure and after 3 days monitored via an inverted microscopy. RESULTS: HEAML selectively inhibited proliferation of HSF-PI-16 cells at higher concentration (>20.0 mg/mL), and stimulated at lower concentrations (<20.0 mg/mL). Following, HSF-PI-16 media treatments up to 72 h, HEAML demonstrated significantly elevated proliferation rates (p<0.05) and stimulation in a scratch wound assay (p<0.04). Furthermore, the morphological analysis of HSF-PI-16 cells at culture media were detected the figures of round to spindle, non-adherent, immature and mature cells. CONCLUSION: These results clearly demonstrate the absence of any toxic effect of HEAML on human skin fibroblasts. To the best of our knowledge, this is the first report elucidating potential mechanisms of action of HEAML on fibroblasts proliferation, and stimulation, offering a greater insight and a better understanding of its effect in future studies.

Item Type: Article
Creators:
CreatorsEmail
Ghobadian, Z.UNSPECIFIED
Ahmadi, M. R.UNSPECIFIED
Rezazadeh, L.UNSPECIFIED
Hosseini, E.UNSPECIFIED
Kokhazadeh, T.UNSPECIFIED
Ghavam, S.UNSPECIFIED
Keywords: Achillea millefolium Culture Fibroblasts Proliferation Skin plant extract Achillea cell proliferation chemistry drug effect fibroblast human in vitro study plant leaf plant stem Humans In Vitro Techniques Plant Extracts Plant Leaves Plant Stems
Divisions:
Page Range: pp. 212-217
Journal or Publication Title: Medical archives (Sarajevo, Bosnia and Herzegovina)
Journal Index: Scopus
Volume: 69
Number: 4
Identification Number: https://doi.org/10.5455/medarh.2015.69.212-217
ISSN: 0350199X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1447

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