Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Novel antiviral activity of mung bean sprouts against respiratory syncytial virus and herpes simplex virus -1: An in vitro study on virally infected Vero and MRC-5 cell lines

Sat Jun 25 09:49:52 2022

(2015) Novel antiviral activity of mung bean sprouts against respiratory syncytial virus and herpes simplex virus -1: An in vitro study on virally infected Vero and MRC-5 cell lines. Bmc Complementary and Alternative Medicine. ISSN 14726882 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: New sources for discovering novel antiviral agents are desperately needed. The current antiviral products are both expensive and not very effective. Methods: The antiviral activity of methanol extract of mung bean sprouts (MBS), compared to Ribavarin and Acyclovir, on respiratory syncytial virus (RSV) and Herpes Simplex virus -1 (HSV-1) was investigated using cytotoxicity, virus yield reduction, virucidal activity, and prophylactic activity assays on Vero and MRC-5 cell lines. Moreover, the level of antiviral cytokines, IFNβ, TNFaα, IL-1, and IL-6 was assessed in MBS-treated, virally infected, virally infected MBS-treated, and control groups of MRC-5 cells using ELISA. Results: MBS extract showed reduction factors (RF) 2.2 × 10 and 0.5 × 102 for RSV and HSV-1, respectively. The 2 h incubation virucidal and prophylactic selectivity indices (SI) of MBS on RSV were 14.18 and 12.82 versus Ribavarin SI of 23.39 and 21.95, respectively, and on HSV-1, SI were 18.23 and 10.9 versus Acyclovir, 22.56 and 15.04, respectively. All SI values were >10 indicating that MBS has a good direct antiviral and prophylactic activities on both RSV and HSV-1. Moreover, interestingly, MBS extract induced vigorously IFNβ, TNFaα, IL-1, and IL-6 cytokines in MRC-5 infected-treated group far more than other groups (P < 0.05) and induced TNFaα and IL-6 in treated group more than infected group (P < 0.05). Conclusions: MBS extract has potent antiviral and to a lesser extent, prophylactic activities against both RSV and HSV-1, and in case of HSV-1, these activities were comparable to Acyclovir. Part of the underlying mechanism(s) of these activities is attributed to MBS potential to remarkably induce antiviral cytokines in human cells. Hence, we infer that MBS methanol extract could be used as such or as purified active component in protecting and treating RSV and HSV-1 infections. More studies are needed to pinpoint the exact active components responsible for the MBS antiviral activities. © 2015 Hafidh et al.

Item Type: Article
Creators:
CreatorsEmail
Hafidh, R. R.UNSPECIFIED
Abdulamir, A. S.UNSPECIFIED
Abu Bakar, F.UNSPECIFIED
Sekawi, Z.UNSPECIFIED
Jahansheri, F.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Keywords: Acyclovir Cytotoxicity assay Herpes simplex virus-1 Interferon Mung bean sprout Respiratory syncytial virus Ribavarin Tumor necrosis factor aciclovir beta interferon interleukin 1 interleukin 6 methanol Mung bean extract plant extract ribavirin tumor necrosis factor alpha unclassified drug antivirus agent cytokine animal cell antiviral activity Article controlled study cytopathogenic effect drug cytotoxicity embryo enzyme linked immunosorbent assay herpes simplex Herpes simplex virus human human cell Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection Human respiratory syncytial virus in vitro propagation in vitro study incubation time lung fibroblast monolayer culture mung bean nonhuman respiratory syncytial virus infection sprout supernatant Vero cell line virus inactivation virus neutralization virus replication virus strain virus titration animal Chlorocebus aethiops drug effects Fabaceae Herpes simplex virus 1 metabolism phytotherapy Respiratory Syncytial Virus Infections Simplexvirus virology Animals Antiviral Agents Cercopithecus aethiops Cytokines Herpesvirus 1, Human Humans In Vitro Techniques Plant Extracts Respiratory Syncytial Viruses Vero Cells
Divisions:
Journal or Publication Title: Bmc Complementary and Alternative Medicine
Journal Index: Scopus
Volume: 15
Number: 1
Identification Number: https://doi.org/10.1186/s12906-015-0688-2
ISSN: 14726882 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1435

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