Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR

Tue Oct 4 14:24:08 2022

(2016) Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR. Asian Pacific Journal of Tropical Disease. pp. 347-349. ISSN 22221808 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Objective: To detect Toxoplasma gondii (T. gondii) among end-stage renal disease (ESRD) patients. Methods: This case-control study was conducted on 180 blood samples. In compliance with all ethical principles, 90 blood samples were taken from hemodialysis patients with ESRD and 90 samples from healthy volunteers. T. gondii screening was done using ELISA to search for immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies and by PCR for amplification of the T. gondii genome using specific primers. Results: The results were analyzed using SPSS software. Out of 90 patients on hemodialysis, 54 (60.0) were positive for anti-toxoplasma IgG antibody, 3 (3.3) for anti-toxoplasma IgM antibody and 5 patients (6) were positive by PCR. From 90 healthy volunteers, 34 (37.8) were positive for anti-toxoplasma IgG antibody. All the healthy volunteers were negative for anti-toxoplasma IgM antibody and in PCR. Compared with the gold standard method of ELISA, PCR had 100 sensitivity and 98.9 specificity in detection of T. gondii. Conclusions: PCR alongside serologic methods can be valuable for T. gondii screening. Given the high prevalence of T. gondii among hemodialysis patients with ESRD, T. gondii screening together with sanitary control of biological agents was recommended in dialysis units. © 2016 Asian Pacific Tropical Medicine Press.

Item Type: Article
Creators:
CreatorsEmail
Rezavand, B.UNSPECIFIED
Poornaki, A. M.UNSPECIFIED
Mokhtari, K. R.UNSPECIFIED
Mohammad, A.UNSPECIFIED
Andalibian, A.UNSPECIFIED
Abdi, J.UNSPECIFIED
Keywords: Diagnosis ELISA Opportunistic infections PCR Toxoplasma gondii immunoglobulin G antibody immunoglobulin M antibody parasite antibody adult Article case control study controlled study diagnostic test accuracy study end stage renal disease enzyme linked immunosorbent assay female gold standard hemodialysis patient human intermethod comparison major clinical study male parasite identification polymerase chain reaction prevalence priority journal sensitivity and specificity toxoplasmosis
Divisions:
Page Range: pp. 347-349
Journal or Publication Title: Asian Pacific Journal of Tropical Disease
Journal Index: Scopus
Volume: 6
Number: 5
Identification Number: https://doi.org/10.1016/S2222-1808(15)61044-1
ISSN: 22221808 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1366

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