Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Correlation between qacE and qacEΔ1 efflux pump genes, antibiotic and disinfectant resistant among clinical isolates of E.Coli

Fri Sep 30 01:18:53 2022

(2016) Correlation between qacE and qacEΔ1 efflux pump genes, antibiotic and disinfectant resistant among clinical isolates of E.Coli. Recent patents on anti-infective drug discovery. pp. 189-195. ISSN 1574891X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction: Antiseptics and disinfectants have been used widely in hospitals and other health care settings to control the growth of microorganisms. However, some disinfectant resistant strains were reported. The objectives of our study were to evaluate correlation between the efflux pump genes, drugs and disinfectant resistant among clinical isolates of E.coli. Methods: A total of 102 of E. coli strains were isolated from urine sample of hospitalized patients. The antibiotic susceptibility was carried out by disc diffusion method. Didecyl di-methyl ammonium chloride (DDDMAC) was used as Quaternary ammonium compound (QAC) disinfectant which was used in Heart Center Hospital. PCR reaction was carried out for detection of qacE and qacΔE efflux pump genes. Result: Almost all the strains had higher resistance to ampicillin, ciproflaxacin, cotrimaxazole and cephalothin. Totally 49 (n: 50) of strains were produced ESBL. Almost all the strains have MIC value between 0.00195 to 0.0078 mg/l for DDDMAC. Correlation between presence of qacE and qacΔE genes and antibiotic resistance was perceived. Presence of qacE and qacΔE genes among strains that have high disinfectant MIC value were 96.9 and 93.7 respectively. In addition, 98 of ESBL producing strains harbored qacE gene and 94 of ESBL producing strains harbored qacΔE gene. Conclusion: Our study indicated that there was a strong correlation between presence of qacE and qacΔE genes with resistance to some antibiotics and growth in media which contain high concentration of disinfectant. In conclusion, other mechanisms also play important role in resistant to antimicrobial agents but the role of efflux pumps in resistant to antimicrobial agents should not be neglected. © 2016 Bentham Science Publishers.

Item Type: Article
Creators:
CreatorsEmail
Shafaati, M.UNSPECIFIED
Boroumand, M.UNSPECIFIED
Nowroozi, J.UNSPECIFIED
Amiri, P.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Keywords: DDDMAC Disinfectant Efflux pump QAC amoxicillin plus clavulanic acid ampicillin aztreonam cefalotin cefoxitin ceftazidime ceftriaxone ciprofloxacin cotrimoxazole disinfectant agent gentamicin imipenem meropenem nitrofurantoin quaternary ammonium derivative antiinfective agent carrier protein Escherichia coli protein antibiotic sensitivity Article bacterial gene bacterium detection bacterium isolate cross-sectional study disinfection disk diffusion Escherichia coli Escherichia coli infection genetic analysis minimum inhibitory concentration nonhuman phenotype polymerase chain reaction priority journal qacE delta1 efflux pump gene qacE efflux pump gene antibiotic resistance drug effects genetics human metabolism microbial sensitivity test procedures Anti-Bacterial Agents Cross-Sectional Studies Disinfectants Drug Resistance, Bacterial Escherichia coli Proteins Humans Membrane Transport Proteins Microbial Sensitivity Tests
Divisions:
Page Range: pp. 189-195
Journal or Publication Title: Recent patents on anti-infective drug discovery
Journal Index: Scopus
Volume: 11
Number: 2
Identification Number: https://doi.org/10.2174/1574891X11666160815094718
ISSN: 1574891X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1353

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