Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The phylogenetic similarity of hydatid cyst isolated from humans and sheep in Ilam Province southwest of Iran

Thu Jun 20 16:40:53 2024

(2016) The phylogenetic similarity of hydatid cyst isolated from humans and sheep in Ilam Province southwest of Iran. Comparative Clinical Pathology. pp. 1221-1226. ISSN 16185641 (ISSN)

Full text not available from this repository.

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Hydatid cyst is a chronic zoonotic disease caused by the larval stage of the dog tapeworm, Echinococcus granulosus. To identify genotype of hydatid cysts of human and sheep jackal in Ilam Province (South West of Iran), the PCR-RFLP and DNA sequencing were used. A total of 10 human and 20 sheep protoscoleces hydatid cyst samples were collected from different hospitals and slaughterhouses. Then, the gene of cox1 of mitDNA of the parasite was amplified and PCR products were cut using AluI and HpaII restriction enzymes. Finally, a number of PCR products were bi-directionally sequenced. Based on the DNA sequencing and PCR-RFLP results, human and sheep samples indicated to pertain the genotypic similarities. Our data indicated that, the genotypes of larval stage of E. granulosus is similar in both intermediate hosts. According to the phylogenetic tree, there is at least one genotype of parasite, which belongs to E. granulosous sensu stricto (G1–G3) complex and overall isolates sequences of mtDNA indicated 100 homology with references G1, G2, and G3 sequences in the GenBank database. G1 genotype was the dominant genotype of human and livestock. © 2016, Springer-Verlag London.

Item Type: Article
Creators:
CreatorsEmail
Shamsi, M.UNSPECIFIED
Dalimi, A.UNSPECIFIED
Khosravi, A.UNSPECIFIED
Ghafarifar, F.UNSPECIFIED
Keywords: Echinococcus granulosus Human Ilam Iran Sheep
Divisions:
Page Range: pp. 1221-1226
Journal or Publication Title: Comparative Clinical Pathology
Journal Index: Scopus
Volume: 25
Number: 6
Identification Number: https://doi.org/10.1007/s00580-016-2332-z
ISSN: 16185641 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1346

Actions (login required)

View Item View Item