Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Comparative Assessment of Induced Immune Responses Following Intramuscular Immunization with Fusion and Cocktail of LeIF, LACK and TSA Genes Against Cutaneous Leishmaniasis in BALB/c Mice

Sat Jul 20 20:34:49 2024

(2018) Comparative Assessment of Induced Immune Responses Following Intramuscular Immunization with Fusion and Cocktail of LeIF, LACK and TSA Genes Against Cutaneous Leishmaniasis in BALB/c Mice. Archivum Immunologiae Et Therapiae Experimentalis. pp. 55-64. ISSN 0004-069X

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

In the present study, we evaluated induced immune responses following DNA vaccine containing cocktail or fusion of LeIF, LACK and TSA genes or each gene alone. Mice were injected with 100 lg of each plasmid containing the gene of insert, plasmid DNA alone as the first control group or phosphate buffer saline as the second control group. Then, cellular and humoral responses, lesion size were measured for all groups. All vaccinated mice induced Th1 immune responses against Leishmania characterized by higher IFN-gamma and IgG2a levels compared with control groups (p<0.05). In addition, IFN-gamma levels increased in groups immunized with fusion and cocktail vaccines in comparison with LACK (p<0.001) and LeIF (p<0.01) groups after challenge. In addition, fusion and cocktail groups produced higher IgG2a values than groups vaccinated with a gene alone (p<0.05). Lesion progression delayed for all immunized groups compared with control groups from 5th week post-infection (p<0.05). Mean lesion size decreased in immunized mice with fusion DNA than three groups vaccinated with one gene alone (p< 0.05). While, lesion size decreased significantly in cocktail recipient group than LeIF recipient group (p< 0.05). There was no difference in lesion size between fusion and cocktail groups. Overall, immunized mice with cocktail and fusion vaccines showed stronger Th1 response by production of higher IFN-gamma and IgG2a and showed smaller mean lesion size. Therefore, use of multiple antigens can improve induced immune responses by DNA vaccination.

Item Type: Article
Creators:
CreatorsEmail
Maspi, N.UNSPECIFIED
Ghaffarifar, F.UNSPECIFIED
Sharifi, Z.UNSPECIFIED
Dalimi, A.UNSPECIFIED
Dayer, M. S.UNSPECIFIED
Keywords: Leishmania Vaccine Immunization Fusion Cocktail thiol-specific antioxidant DNA vaccines polyprotein vaccine visceral leishmaniasis major infection t-cells protection expression antigen leishmanization Immunology
Divisions:
Page Range: pp. 55-64
Journal or Publication Title: Archivum Immunologiae Et Therapiae Experimentalis
Journal Index: ISI
Volume: 66
Number: 1
Identification Number: https://doi.org/10.1007/s00005-017-0484-4
ISSN: 0004-069X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/134

Actions (login required)

View Item View Item