Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins

Tue Jul 23 03:08:58 2024

(2017) Inhibitory effect of luteolin on MKN-45 tumorg gastric cells: The role of nucleoside-diphosphate kinases, cofilin and actin proteins. Koomesh. pp. 611-618. ISSN 16087046 (ISSN)

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Introduction: Luteolin is an important plant extract. This shows anti-inflammatory, inhibition of cell growth, induction of apoptosis and inhibition of metastasis. Gastric cancer is one of the most common cancers in the world. Also metastasis is widespread in this kind of cancer and late diagnosis is a cause of patient's death. Cofilin, actin andnucleoside-diphosphate kinases (NDPK) are important in cell death and metastasis. In this study the effect of luteolin on MKN-45 gastric carcinoma cells was evaluated. Materials and Methods: At first the cells were cultured and then for 48 hours were cultured with the drug concentration. Then the cells where collected and their protein were extracted and analyzed using 2D electrophoresis and separated on the basis of their molecular mass and PI. After that these samples were used for determine the amino acid sequence by MOLDI-TOF. Results: The growth rate of MKN-45 inhibited under influence of luteolin, this means increasing Apoptosis. Based on the results presented here by identification of protein spots by MOLDI-TOF mass spectrometry the expression of three protein cofilin, actin and NDPK has been decreased by luteolin. Conclusion: It seems that the anti- tumoral effect of luteolin is expressed through three proteins cofilin, actin and NDPK in gastric cancer cells. © 2017, Semnan University of Medical Sciences. All rights reserved.

Item Type: Article
Keywords: Actins Cofilin Electrophoresis Luteolin Nucleoside-Diphosphate Kinase Stomach Neoplasms actin nucleoside diphosphate kinase amino acid sequence antineoplastic activity apoptosis Article cancer inhibition cell culture gastric cancer cell line human human cell matrix assisted laser desorption ionization time of flight mass spectrometry metastasis protein expression stomach carcinoma stomach tumor two dimensional electrophoresis
Page Range: pp. 611-618
Journal or Publication Title: Koomesh
Journal Index: Scopus
Volume: 19
Number: 3
ISSN: 16087046 (ISSN)
Depositing User: مهندس مهدی شریفی

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