Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Ethanolic Extract of Berberis Vulgaris Fruits Inhibits the Proliferation of MCF-7 Breast Cancer Cell Line Through Induction of Apopto

Thu Apr 18 23:34:19 2024

(2017) Ethanolic Extract of Berberis Vulgaris Fruits Inhibits the Proliferation of MCF-7 Breast Cancer Cell Line Through Induction of Apopto. Infectious Disorders - Drug Targets. pp. 192-198. ISSN 18715265 (ISSN)

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Background: As it is obvious, there is much documentation that shows the importance of breast cancer treatment in patients. High expressions of P53 and Bcl-2 are associated with breast cancer, which are reliable factors to follow up the breast cancer. Berberis vulgaris is used as a traditional medicine in cancer. Despite of the fact that many researches have demonstrated its anti-cancer properties, there are no scientific documents to show its efficacy in detail in breast cancer. Objective: Because of traditional use of B.vulgaris and little knowledge about its effects, our research was focused on determining the efficacy and toxicity of B. vulgaris. For this reason, we determined the efficacy of B. vulgaris on breast cancer cells. Method: As described in Method section, standard protocols including MTT assay and qPCR were performed to identify the effect of B. vulgaris ethanolic extract against breast cancer cells. Results: Our results clearly demonstrated that 35 mg/ml had IC50 against 3t3 normal cells, and 9 mg/ml of B. vulgaris was effective against MCF-7 breast cancer cells. The results demonstrated that even at only 1 mg/ml concentration of B. vulgaris, crude extract was effective, 9 mg/ml and 12 mg/ml of extract had better anti-cancer activity compared with doxorubicin. Conclusion: Despite that the role of anticancer properties of B. vulgaris was clearly defined in some patents, our results demonstrated the potency of B. vulgaris against breast cancer, but further analysis should be performed to candidate this herb as an anti-breast cancer drug. © 2017 Bentham Science Publishers.

Item Type: Article
Keywords: Bcl-2 Berberis vulgaris Breast neoplasms Crude extract MCF-7 p53 doxorubicin protein p53 antineoplastic agent BCL2 protein, human plant extract protein bcl 2 TP53 protein, human Article bioinformatics breast cancer cell viability assay DNA extraction drug efficacy enzyme linked immunosorbent assay MTT assay nonhuman polymerase chain reaction protein expression Pseudomonas aeruginosa real time polymerase chain reaction RNA extraction 3T3 cell line animal apoptosis Berberis breast tumor cell proliferation down regulation drug effect female fruit gene expression genetics human MCF-7 cell line mouse pathology phytotherapy 3T3 Cells Animals Antineoplastic Agents, Phytogenic Down-Regulation Humans MCF-7 Cells Mice Plant Extracts Proto-Oncogene Proteins c-bcl-2 Tumor Suppressor Protein p53
Page Range: pp. 192-198
Journal or Publication Title: Infectious Disorders - Drug Targets
Journal Index: Scopus
Volume: 17
Number: 3
Identification Number:
ISSN: 18715265 (ISSN)
Depositing User: مهندس مهدی شریفی

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