Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Prevalence of hepatitis B virus in chronic hepatitis B patients

(2012) Prevalence of hepatitis B virus in chronic hepatitis B patients. Roumanian archives of microbiology and immunology. pp. 53-5. ISSN 1222-3891 (Print) 1222-3891 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/22838221

Abstract

The aim of the current study was to detect HBV by Real time - PCR in chronic hepatitis B patients. Fifty-eight sera of chronic hepatitis B patients were subjected during the period March 2009 to April 2010 in Ilam cities in West of Iran. Sera assayed by real-time PCR and ELISA methods. Twenty serum samples from healthy volunteers and non-hepatitis B patients and negative for hepatitis B seromarkers served as negative controls for the study. Among fifty-eight sera, ELISA showed fifty-five (94.8) of the samples were positive for HBsAg and three (5.2) negative results obtained while real-time PCR specified fifty-eight (100) positive results in chronic hepatitis B patients. HBsAg status did not necessarily reflect HBV DNA level in the serum, as 5.2 of chronic Hepatitis B patients were positive for HBV DNA but negative for HBsAg. HBV DNA was not found to be positive amongst any of the negative controls. Real time - PCR is a sensitive and reproducible assay for HBV DNA quantization.

Item Type: Article
Keywords: DNA, Viral/analysis Hepatitis B Surface Antigens/analysis/genetics Hepatitis B virus/genetics/*isolation & purification Hepatitis B, Chronic/*virology Humans Prevalence
Page Range: pp. 53-5
Journal or Publication Title: Roumanian archives of microbiology and immunology
Journal Index: Pubmed
Volume: 71
Number: 1
ISSN: 1222-3891 (Print) 1222-3891 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1187

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