Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Contribution of AcrAB efflux pump to ciprofloxacin resistance in Klebsiella pneumoniae isolated from burn patients

Sun Aug 14 01:35:16 2022

(2013) Contribution of AcrAB efflux pump to ciprofloxacin resistance in Klebsiella pneumoniae isolated from burn patients. Gms Hygiene and Infection Control. Doc15. ISSN 2196-5226 (Print) 2196-5226 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/24327941

Abstract

Resistance to fluoroquinolones has been recently increased among bacterial strains isolated from outpatients. Multidrug-resistant K. pneumoniae is one of the major organisms isolated from burn patients and the AcrAB efflux pump is the principal pump contributing to the intrinsic resistance in K. pneumoniae against multiple antimicrobial agents including ciprofloxacin and other fluoroquinolones. Fifty-two K. pneumoniae isolated from burn patients in Shahid Motahari hospital and confirmed by conventional biochemical tests. Antimicrobial susceptibility testing was done according to CLSI 2011 guidelines, to determine the antimicrobial resistance pattern of isolates. AcrA gene was detected among ciprofloxacin-resistant isolates by PCR assay. MICs to ciprofloxacin were measured with and without carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Forty out of the 52 K. pneumoniae isolated from burn patients in Shahid Motahari hospital were resistant to ciprofloxacin according to breakpoint of CLSI guideline. PCR assay for acrA gene demonstrated that all ciprofloxacin-resistant isolates harbored acrA gene coding the membrane fusion protein AcrA and is a part of AcrAB efflux system. Among these isolates, 19 strains (47.5) showed 2 to 32 fold reduction in MICs after using CCCP as an efflux pump inhibitor. The other 21 strains (52.5) showed no disparity in MICs before and after using CCCP. In conclusion, the AcrAB efflux system is one of the principal mechanisms contribute in ciprofloxacin resistance among K. pneumoniae isolates but there are some other mechanisms interfere with ciprofloxacin resistance such as mutation in target proteins of DNA gyrase of topoisomerase IV enzymes.

Item Type: Article
Creators:
CreatorsEmail
Pakzad, I.UNSPECIFIED
Zayyen Karin, M.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Boustanshenas, M.UNSPECIFIED
Lari, A. R.UNSPECIFIED
Keywords: Klebsiella pneumoniae ciprofloxacin resistance efflux system
Divisions:
Page Range: Doc15
Journal or Publication Title: Gms Hygiene and Infection Control
Journal Index: Pubmed
Volume: 8
Number: 2
Identification Number: https://doi.org/10.3205/dgkh000215
ISSN: 2196-5226 (Print) 2196-5226 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1163

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