Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Detection of highly ciprofloxacin resistance acinetobacter baumannii isolated from patients with burn wound infections in presence and absence of efflux pump inhibitor

Thu Aug 11 17:08:49 2022

(2014) Detection of highly ciprofloxacin resistance acinetobacter baumannii isolated from patients with burn wound infections in presence and absence of efflux pump inhibitor. Maedica. pp. 162-7. ISSN 1841-9038 (Print) 1841-9038 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/25705272

Abstract

The emergence of fluoroquinolone resistance among A. baumannii isolates is now of particular concern. Phenotypic and genotypic characteristics of resistance to ciprofloxacin among 50 Acinetobacter baumannii isolated from burn wound infections of Tehran were evaluated by E-test and broth microdilution in presence and absence of efflux pump inhibitor phenylalanine- arginine beta-naphthylamide (PAbetaN) and PCR-sequencing methods. All isolates were then typed by REP-PCR fingerprinting to find the clonal relationship between resistant isolates. Our results indicated that resistance to ciprofloxacin among A. baumannii isolated from burn infections in Tehran are high with resistance rate of 100 and ciprofloxacin resistant isolates have a mutation of Serine 83 —>Leucine in the quinolone resistance determining region (QRDR) of DNA gyrase subunit A (GyrA). 38 of the isolates showed MIC ranges of 64 to >/=512mug/ml and were considered as highly resistant. We could not detect Par C mutations and plasmid-mediated quinolone resistance A (qnrA) among ciprofloxacin resistant isolates. When we used the efflux pump inhibitor PAbN, MIC of ciprofloxacin was reduced two-to four folds. REP-type A (25/50; 50), B (20/50; 30) and C (10/50; 20) were the most common REP-types among A. baumannii isolates. It seems that mutation in GyrA is the main mechanism of resistant to ciprofloxacin among A. baumannii isolates from burn infections and presence of efflux pumps is just secondary target for ciprofloxacin resistant among A. baumannii in Iran. Regarding with limitation of REP-types detected in this study, we found good correlation between resistance to ciprofloxacin and REP-types A-C.

Item Type: Article
Creators:
CreatorsEmail
Maleki, M. H.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Khayat, H.UNSPECIFIED
Mohammadi, M.UNSPECIFIED
Pourahmad, F.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Soroush, S.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Keywords: A. baumannii ciprofloxacin resistance gyr A QRDRS
Divisions:
Page Range: pp. 162-7
Journal or Publication Title: Maedica
Journal Index: Pubmed
Volume: 9
Number: 2
ISSN: 1841-9038 (Print) 1841-9038 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1109

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