Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Diversity of virulence genes in Brucella melitensis and Brucella abortus detected from patients with rheumatoid arthritis

Wed May 18 18:14:32 2022

(2018) Diversity of virulence genes in Brucella melitensis and Brucella abortus detected from patients with rheumatoid arthritis. Microbial Pathogenesis. pp. 247-250. ISSN 0882-4010

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

The presence of Brucella melitensis and Brucella abortus genomes were investigated in the synovial fluid (SF) samples from 90 patients with rheumatoid arthritis (RA). DNA extraction and PCR assay were performed for simultaneous identification and discrimination of B. melitensis and B. abortus from the SF using three specific primers. After gel electrophoresis, the PCR products were confirmed by DNA sequencing. The cbg, omp31, manA, virB, and znuA virulence genes typing were performed by multiplex-PCR. Of the 90 samples, 14 were positive for B. melitensis (n = 9; 10) and B. abortus (n = 5; 5.5). The virulotyping of positive samples revealed the presence of all five virulence genes in B. melitensis. The virB, cbg, and otn31 were detected in all five samples of B. abortus. In addition, zhuA and manA were detected in three (60) and four (80) samples, respectively, of the B. abortus-positive samples. Moreover, a total of 94.2 and 89.2 of the 14 positive samples were also found positive for manA and znuA, respectively. Our findings revealed that the Brucella spp. genomes can be detected in the SF of RA patients by the PCR-based method. We thus suggest that physicians should consider the Brucella spp. as indicators of potential RA for the timely diagnosis and treatment of RA.

Item Type: Article
Creators:
CreatorsEmail
Rahdar, H. A.UNSPECIFIED
Golmohammadi, R.UNSPECIFIED
Mirnejad, R.UNSPECIFIED
Ataee, R. A.UNSPECIFIED
Alishiri, G. H.UNSPECIFIED
Kazemian, H.UNSPECIFIED
Keywords: Brucella melitensis Brucella abortus Synovial fluid Rheumatoid arthritis Virulotyping PCR intracellular survival system protein spp. pcr Immunology Microbiology
Divisions:
Page Range: pp. 247-250
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 118
Identification Number: https://doi.org/10.1016/j.micpath.2018.03.034
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/88

Actions (login required)

View Item View Item