Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Human wild-type superoxide dismutase 1 gene delivery to rat bone marrow stromal cells: its importance and potential future trends

Sat May 28 11:54:52 2022

(2018) Human wild-type superoxide dismutase 1 gene delivery to rat bone marrow stromal cells: its importance and potential future trends. Iranian Journal of Basic Medical Sciences. pp. 688-694. ISSN 2008-3866

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Objective(s): Human superoxide dismutase 1 (SOD1) is the cytosolic form of this enzyme it detoxifies superoxide anions and attenuates their toxicities and concomitant detrimental effects on the cells. It is believed that the amount of these enzymes present in the oxidative stress-induced diseases is crucial for preventing disease progression. Transfection of rat bone marrow stromal cells (BMSCs) by a constructed vector carrying the human wild-type SOD1 gene, a non-viral gene transfer method, was the main aim of this study. Materials and Methods: For this purpose, the rat BMSCs were transfected with the vector using Turbofect reagent and then stabilized. Western-blot and real-time PCR were also used for evaluation of SOD1 expression. Results: Data analysis from RT-PCR and Western-blot techniques revealed that the stable transfected cells could secrete human wild-type SOD1 in the supernatant. Also, the total activity of SOD1 was about 0.5 +/- 0.09 U/ml and 0.005 +/- 0.002 U/ml in the supernatants of the transfected and not-transfected of rat BMSCs, respectively. Conclusion: This study showed that expansion of the stable transfected rat BMSCs by a constructed vector carrying the human wild-type SOD1 gene is capable of secreting the active SOD1 enzyme under ex-vivo conditions. The recommendation of this study is that the same experiment would be applicable for expression of the other form of this enzyme, SOD3, as well. More valuable information could probably be provided about the variety of the diseases caused by superoxide anions toxicities by intervention and application of the non-viral method for expressions of SOD1 and SOD3 enzymes.

Item Type: Article
Creators:
CreatorsEmail
Abedi, M.UNSPECIFIED
Mesbah-Namin, S. A.UNSPECIFIED
Noori-Zadeh, A.UNSPECIFIED
Tiraihi, T.UNSPECIFIED
Taheri, T.UNSPECIFIED
Keywords: Expression Ex-vivo Gene delivery Human SOD1 Rat BMSCs Vector amyotrophic-lateral-sclerosis transgenic mice endothelial dysfunction neurotrophic factors parkinsons-disease oxidative stress apoptotic death animal-model expression neurons Research & Experimental Medicine Pharmacology & Pharmacy
Divisions:
Page Range: pp. 688-694
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: ISI
Volume: 21
Number: 7
ISSN: 2008-3866
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/60

Actions (login required)

View Item View Item