Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Controlled surface morphology and hydrophilicity of polycaprolactone toward selective differentiation of mesenchymal stem cells to neural like cells

Wed May 18 17:26:28 2022

(2015) Controlled surface morphology and hydrophilicity of polycaprolactone toward selective differentiation of mesenchymal stem cells to neural like cells. Journal of Biomedical Materials Research Part A. pp. 1875-1881. ISSN 1549-3296

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Differentiation of mesenchymal stem cells (MSCs) into neuron cells has great potential in therapy of damaged nerve tissue. It has been shown that three-dimensional biomaterials have great ability to up regulate the expression of neuronal proteins. In this study, O-2 plasma technology was used to enhance hydrophilicity of poly (epsilon-caprolactone) (PCL) toward selective differentiation of MSCs into neural cells. Random and aligned PCL nanofibers scaffolds were fabricated by electrospinning method and their physicochemical and mechanical properties were carried out by scanning electron microscope (SEM), contact angle, and tensile measurements. Contact angle studies of PCL and plasma treated PCL (p-PCL) nanofibers revealed significant change on the surface properties PCL nanofibers from the view point of hydrophilicity. Physiochemical studies revealed that p-PCL nanofibers were extremely hydrophilic compared with untreated PCL nanofibers which were highly hydrophobic and nonabsorbent to water. Differentiation of MSCs were carried out by inducing growth factors including basic fibroblast growth factor, nerve growth factor, and brain derived growth factor, NT3, 3-isobutyl-1-methylxanthine (IBMX) in Dulbecco's modified Eagle's medium/F12 media. Differentiated MSCs on nanofibrous scaffold were examined by immunofluorescence assay and was found to express the neuronal proteins; -tubulin III and Map2, on day 15 after cell culture. The real-time polymerase chain reaction (RT-PCR) analysis showed that p-PCL nanofibrous scaffold could upregulate expression of Map-2 and downregulate expression of Nestin genes in nerve cells differentiated from MSCs. This study indicates that mesenchymal stem cell cultured on nanofibrous scaffold have potential differentiation to neuronal cells on and could apply in nerve tissue repair. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1875-1881, 2015.

Item Type: Article
Creators:
CreatorsEmail
Jahani, H.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Wu, C. Y.UNSPECIFIED
Kaviani, S.UNSPECIFIED
Soleimani, M.UNSPECIFIED
Abassi, N.UNSPECIFIED
Ou, K. L.UNSPECIFIED
Hosseinkhani, H.UNSPECIFIED
Keywords: mesenchymal stem cells tissue engineering hydrophilicity surface modification neural cells marrow stromal cells peptide-amphiphile nanofibers ectopic bone-formation in-vitro osteogenic differentiation perfusion culture fibrous scaffolds proliferation regeneration fabrication Engineering Materials Science
Divisions:
Page Range: pp. 1875-1881
Journal or Publication Title: Journal of Biomedical Materials Research Part A
Journal Index: ISI
Volume: 103
Number: 5
Identification Number: https://doi.org/10.1002/jbm.a.35328
ISSN: 1549-3296
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/580

Actions (login required)

View Item View Item