Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Application of natural compounds–based gold nanoparticles for the treatment of hemolytic anemia in an anemic mouse model: Formulation of a novel drug from relationship between the nanotechnology and hematology sciences

Mon May 23 15:37:12 2022

(2020) Application of natural compounds–based gold nanoparticles for the treatment of hemolytic anemia in an anemic mouse model: Formulation of a novel drug from relationship between the nanotechnology and hematology sciences. Applied Organometallic Chemistry. ISSN 02682605 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

From time immemorial, people have tried to treat several diseases using natural compounds, including plants. Recently, researchers proposed that plants and herbal nanoparticles possess many remedial potentials. The results of this study confirmed the ability of an aqueous extract of Allium eriophyllum Boiss leaf grown under in vitro conditions for the biosynthesis of gold nanoparticles (AuNPs) and also revealed the anti-hemolytic anemia activity of AuNPs in an anemic rodent model. These nanoparticles were characterized using Fourier-transform infrared spectroscopy (FT-IR) spectroscopy, ultraviolet–visible spectroscopy, X-ray diffraction, field-emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM). TEM and FE-SEM images showed the biosynthesized nanoparticles as having a uniform spherical morphology and diameters in the range of 5–30 nm. In vivo design, the induction of hemolytic anemia, was done using phenylhydrazine in 40 mice. Then, the mice were randomly divided into five groups: HAuCl4, A. eriophyllum, AuNPs, untreated, and control. AuNPs significantly (p ≤ 0.01) decreased the concentration of glucocorticoid receptors in the serum, liver, and spleen, and also alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, total and conjugated bilirubin, cholesterol, triglyceride, low-density lipoprotein, urea, creatinine, ferrous, ferritin, and erythropoietin in the serum increased the concentrations of superoxide dismutase, catalase, and glutathione peroxidase (GPx) in the serum, liver, and spleen and also total protein, high-density lipoprotein, and albumin in serum in the control mice as compared to the anemic mice. Also, AuNPs significantly (p ≤ 0.01) increased the body weight; anti-inflammatory cytokine (IL4, IL5, IL10, IL13, and IFNα) concentration; and the total platelet, white blood cells, lymphocyte, neutrophil, monocyte, eosinophil, and basophil counts and red blood cell parameters but decreased the weight and volume of the liver and spleen and their subcompartments and decreased the concentration of pro-inflammatory cytokines (IL1, IL6, IL12, IL18, IFNY, and TNFα) compared to the untreated mice. In vitro design, using 2,2-diphenyl-1-picrylhydrazyl test, revealed similar antioxidant potentials for A. eriophyllum, AuNPs, and butylated hydroxytoluene. In addition, AuNPs were similar to A. eriophyllum and had a high cell viability dose dependently against the human umbilical vein endothelial cell line. In conclusion, the results of the chemical characterization confirm that the leaves of A. eriophyllum can be used to produce AuNPs with a remarkable amount of anti-hemolytic anemia property. © 2020 John Wiley & Sons, Ltd.

Item Type: Article
Creators:
CreatorsEmail
Goorani, S.UNSPECIFIED
Koohi, M. K.UNSPECIFIED
Morovvati, H.UNSPECIFIED
Hassan, J.UNSPECIFIED
Ahmeda, A.UNSPECIFIED
Zangeneh, M. M.UNSPECIFIED
Keywords: Allium eriophyllum Boiss anemic mouse model gold nanoparticles hemolytic anemia phenylhydrazine Alkalinity Amino acids Biochemistry Blood Cell culture Controlled drug delivery Drug products Enamels Endothelial cells Fiber optic sensors Field emission microscopes Fourier transform infrared spectroscopy High resolution transmission electron microscopy Lipoproteins Mammals Metal nanoparticles Phosphatases Plants (botany) Scanning electron microscopy Urea 2 ,2-diphenyl-1-picrylhydrazyl Field emission scanning electron microscopy Fourier transform infra red (FTIR) spectroscopy Human umbilical vein endothelial cells Mouse models
Divisions:
Journal or Publication Title: Applied Organometallic Chemistry
Journal Index: Scopus
Volume: 34
Number: 4
Identification Number: https://doi.org/10.1002/aoc.5475
ISSN: 02682605 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/2840

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