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Ilam University of Medical Sciences

Distribution of Class D Carbapenemase and Extended-Spectrum beta-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections

Fri May 20 02:30:22 2022

(2017) Distribution of Class D Carbapenemase and Extended-Spectrum beta-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections. Journal of Clinical and Diagnostic Research. DC19-DC23. ISSN 2249-782X

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Abstract

Introduction: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. Aim: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections. Materials and Methods: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A. baumannii using biochemical method, and then identified to species level with PCR of gyrB and blaOXA-51 gene. Antibiotic sensitivity pattern for b-lactam and carbapenem antibiotics was assessed using Agar disc diffusion test and E-test. The presence of different carbapenemase and metalo-b-lactamase (bla(OXA-51-like), gyrB, bla(OXA-23-like), bla(OXA-24-like), bla(OXA-58), bla(VEB), bla(PER), bla(GIM), bla(SIM), bla(IMP), bla(VIM)), extended-spectrum beta-lactamases (blaTEM, blaSHV) and two insertion sequences genes (ISaba1, IS1113) was assessed. Biofilm formation of all isolates was then assessed. Chi-square analysis or Fisher's-exact tests were used for statistical analysis. A p-value < 0.05 was considered statistically significant. Results: Colistin was the most effective antimicrobial agents, although 10.7 (11/ 103) of the isolates were resistant. The high rate of resistance to meropenem (93.2) and imipenem (90.3) was determined. Also, with exception of ampicillin-sulbactam, surprisingly the resistant rate was 28.2, the resistance to b-lactam antibiotic was dramatically increased. Co-existence of two and three blaOXA genes was also determined. The blaOXA58 was detected in only one isolate. The blaTEM and blaOXA23 was the most prevalent Extended-Spectrum beta-Lactamases (ESBL) gene. All isolates were biofilm producers. Conclusion: Antibiotic resistance is increasing among A. baumannii isolates which is due to excessive use of antibiotics and also acquired resistant genes and biofilm production. Resistance to nearly all antimicrobial agents especially colistin as end choice for treatment of multiple drug resistance A. baumannii is a big concern.

Item Type: Article
Creators:
CreatorsEmail
Mohammadi, M.UNSPECIFIED
Soroush, S.UNSPECIFIED
Delfani, S.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Abbaszadeh, A.UNSPECIFIED
Bahmani, M.UNSPECIFIED
Bogdanovic, L.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Keywords: beta-lactams Insertion elements Nosocomial infections intensive-care-unit pseudomonas-aeruginosa antimicrobial resistance biofilm production emergence spp. pcr calcoaceticus prevalence outbreak General & Internal Medicine
Divisions:
Page Range: DC19-DC23
Journal or Publication Title: Journal of Clinical and Diagnostic Research
Journal Index: ISI
Volume: 11
Number: 7
Identification Number: https://doi.org/10.7860/jcdr/2017/25534.10218
ISSN: 2249-782X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/269

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