Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The combination of miR-122 overexpression and Let-7f silencing induces hepatic differentiation of adipose tissue-derived stem cells

Fri May 20 02:19:14 2022

(2017) The combination of miR-122 overexpression and Let-7f silencing induces hepatic differentiation of adipose tissue-derived stem cells. Cell Biology International. pp. 1083-1092. ISSN 1065-6995

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Abstract

Human adipose tissue-derived stem cells (hADSCs) have been considered as a promising source for cell therapy of liver diseases due to their accessibility, abundance, and expression of hepatocyte markers. Currently, the important role of certain microRNAs (miRNAs) has been reported during hepatic differentiation of stem cells. However, the combination effect of miRNAs on hepatic differentiation of these cells needs to be more investigated. The present study seeks to determine whether the combination of miRNAs can enhance hepatic differentiation of hADSCs in the absence of any other stimulation. First, lentiviral transduction was used to overexpress miR-122 and silence let-7f in hADSCs for up to 21 days. Then, hepatic functionality was evaluated by analyzing specific hepatocyte genes and biochemical markers at different time points of differentiation induction. Stable miR-122 overexpression and let-7f silencing together in hADSCs resulted in increased expression of hepatocyte markers including ALB, AFP, CK18, CK19, and HNF4a. In addition, urea and albumin production, immunocytochemistry, and glycogen staining confirmed that the treated cells differentiated toward hepatocyte-like cells. Therefore, our findings demonstrate the possibility of using microRNAs to induce hADSCs into functional hepatocyte-like cells.

Item Type: Article
Creators:
CreatorsEmail
Davoodian, N.UNSPECIFIED
Lotfi, A. S.UNSPECIFIED
Soleimani, M.UNSPECIFIED
Ghaneialvar, H.UNSPECIFIED
Keywords: hepatic differentiation hepatocyte-like cells human adipose tissue-derived stem cells (hADSCs) let-7f miR-122 in-vitro hepatocyte differentiation progenitor cells micrornas expression targets vivo Cell Biology
Divisions:
Page Range: pp. 1083-1092
Journal or Publication Title: Cell Biology International
Journal Index: ISI
Volume: 41
Number: 10
Identification Number: https://doi.org/10.1002/cbin.10836
ISSN: 1065-6995
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/234

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