Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran

Fri May 20 02:16:10 2022

(2017) Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran. Iranian Journal of Parasitology. pp. 614-621. ISSN 1735-7020

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Abstract

Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran. Methods: Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 (nad1) was amplified by PCR then the products were digested by using HpaII, Rsa1, and Alu1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced. Results: Totally, 20 stray dogs out of 75 (26.66) and two out of 73 (2.74) golden jackal showed infection with E. granulosus. Amplified PCR product for all isolates was a band of approximately 550bp. Alu1 digested the product into two bands of approximately 160bp and 390bp fragments, while the Rsa1 cut the product into 320bp and 230bp fragments and the HpaII had no effect on the PCR product for both dog and jackal samples. The isolate sequences of mtDNAnad1 gene indicated 100 homology with references G1, G2 and G3 sequences in the GenBank database. Conclusion: The genotype of adult E. granulosus was similar to larval stage genotypes of parasite (G1-G3 complex).

Item Type: Article
Creators:
CreatorsEmail
Dalimi, A.UNSPECIFIED
Shamsi, M.UNSPECIFIED
Khosravi, A.UNSPECIFIED
Ghaf-Farifar, F.UNSPECIFIED
Keywords: Echinococcus granulosus Stray dog Golden jackal Genotyping Iran genetic-characterization molecular epidemiology mitochondrial genes ardabil province domestic-animals northwest iran cox1 gene dogs livestock strains Parasitology
Divisions:
Page Range: pp. 614-621
Journal or Publication Title: Iranian Journal of Parasitology
Journal Index: ISI
Volume: 12
Number: 4
ISSN: 1735-7020
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/225

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