Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Investigating the effects of rosiglitazone and epigallocatechin-3-gallate on PGC-1α protein expression in palmitate-induced insulin resistant C2C12 skeletal muscle cells

Fri May 20 01:57:03 2022

(2015) Investigating the effects of rosiglitazone and epigallocatechin-3-gallate on PGC-1α protein expression in palmitate-induced insulin resistant C2C12 skeletal muscle cells. Iranian Journal of Endocrinology and Metabolism. pp. 53-62. ISSN 16834844 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction: Alterations in lipids metabolism are one of the important mechanisms for the treatment of insulin resistance and, hence for type 2 diabetes. On the other hand, PGC-1α, as a key regulator of mitochondrial biogenesis and function, by increasing)-oxidation of lipids plays an important role in improving insulin sensitivity. In the present study, the effects of Epigallocatechin- 3-Gallate (EGCG), an anti-obesity and enhancer of lipid catabolism agent, on PGC-1α protein expression was examined and compared with the anti-diabetic drug Rosiglitazone (RGZ). Materials and Methods: After differentiation of C2C12 myoblasts to myotubes, insulin resistance was induced by palmitate treatment. Afterward, PGC-1α protein expression was examined using the western blot method before and after treatment with insulin and after EGCG and RGZ treatment. Results: Palmitate treatment significantly decreased PGC-1 α protein expression in the C2C12 cells (P=0.001). Treatment of these cells with EGCG had no significant effect on the PGC-1α protein expression (P=0.67), whereas treatment with RGZ significantly increased expression of this gene at protein level (P=0.003). In addition, this significant increase in PGC-1α protein expression was maintained by simultaneous treatment with EGCG and RGZ (P=0.001). Conclusion: Our results showed that the effect of EGCG on PGC-1α protein expression was not significant, whereas RGZ significantly improved the palmitate-induced reduction of PGC-1α protein expression. Overall, it seems that anti-diabetic effect of EGCG is not exerted through its effect on the expression of PGC- 1α gene, in contrast to that of RGZ. © 2015, Endocrine Research Center. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Bakhtiyari, S.UNSPECIFIED
Babakhani, A.UNSPECIFIED
Haghani, K.UNSPECIFIED
Keywords: C2C12 muscle cells Epigallocatechin-3-Gallate Insulin resistance PGC-1α gene Rosiglitazone epigallocatechin gallate insulin palmitic acid peroxisome proliferator activated receptor gamma coactivator 1alpha animal cell Article cell differentiation controlled study drug effect mouse muscle cell myoblast myotube nonhuman protein expression skeletal muscle Western blotting
Divisions:
Page Range: pp. 53-62
Journal or Publication Title: Iranian Journal of Endocrinology and Metabolism
Journal Index: Scopus
Volume: 17
Number: 1
ISSN: 16834844 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1477

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