Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In vitro Cytotoxicity Comparison of MTA Fillapex, AH-26 and Apatite Root Canal Sealer at Different Setting Times

Fri May 20 02:15:33 2022

(2017) In vitro Cytotoxicity Comparison of MTA Fillapex, AH-26 and Apatite Root Canal Sealer at Different Setting Times. Iranian endodontic journal. pp. 162-167. ISSN 1735-7497 (Print) 1735-7497 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/28512479

Abstract

INTRODUCTION: This study aimed to compare the cytotoxicity of MTA Fillapex, AH-26 and Apatite root canal sealers at different times after mixing. METHODS AND MATERIALS: In this in vitro study, MTA Fillapex, AH-26 and Apatite root canal sealer were spilled uniformly by 40 microm mesh in a 96-well plate. Then, human fetal foreskin fibroblast cell line (HFFF2) were added to each sealer cell culture medium. Cytotoxicity was measured using MTT assay after 24, 48 and 72 h and seven days. Multiple comparisons were done using analysis of variances (ANOVA) and Scheffe's post hoc test. RESULTS: All studied sealers exhibited severe cytotoxicity (more than 70) except for Apatite sealer (95) at 48 h after mixing. Cytotoxicity of MTA Fillapex and AH-26 were similar (P>0.05) at 24, 48 and 72 h and 7 days after mixing of sealers. Cytotoxicity of MTA Fillapex and Apatite root canal sealer, at 24 and 48 h, were significantly different (P=0.003 and P=0.000, respectively); MTA Fillapex was more cytotoxic. However in 72 h and 7 days after mixing, the difference was not significant (P>0.05). At 24 and 48 h after mixing, AH-26 was more cytotoxic (P=0.002 and P=0.000, respectively). Same as above at 72 h and 7 days after mixing, their cytotoxicity were similar (P>0.05). CONCLUSION: Overall cytotoxicity of all studied materials were severe. However, it was observed that the cytotoxicity of MTA Fillapex, AH-26 and Apatite root canal sealer decreased over time. Apatite root canal sealer exhibited the least cytotoxicity. Cytotoxicity of MTA Fillapex and AH-26 were similar at different time intervals.

Item Type: Article
Creators:
CreatorsEmail
Jafari, F.UNSPECIFIED
Aghazadeh, M.UNSPECIFIED
Jafari, S.UNSPECIFIED
Khaki, F.UNSPECIFIED
Kabiri, F.UNSPECIFIED
Keywords: Cytotoxicity MTT Assay Root Canal Sealer
Divisions:
Page Range: pp. 162-167
Journal or Publication Title: Iranian endodontic journal
Journal Index: Pubmed
Volume: 12
Number: 2
Identification Number: https://doi.org/10.22037/iej.2017.32
ISSN: 1735-7497 (Print) 1735-7497 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1034

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